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Expert Flow Cytometry Services for Phenotypical and Functional Cell Analysis
✔ CAP-accredited & CLIA-certified
✔ GLP Environment
✔ Globally Harmonized
✔ Conventional & Spectral Flow
Speak with a Scientist
From single-cell precision to submission-ready data: one partner, every assay
Our flow cytometry capabilities, including intra-cellular cytokine staining (ICS) and receptor occupancy (RO) assays, are provided by immunologists and flow cytometry experts with many years of experience in customizing assays for clinical trials — including careful optimization of assay parameters to guarantee reliable results.
➜ Multiparametric flow cytometry for exploratory, secondary, or primary clinical endpoints
➜ ICS flow cytometry assays for cytokine testing on fresh whole blood, cryopreserved PBMC, or tumor-infiltrating lymphocytes (TILs)
➜ Custom receptor occupancy assay and receptor binding assay development, or assay transfer to our labs
➜ Regulatory-ready data for FDA, EMA, and beyond — all phases of clinical development
Spectral Flow Cytometry with CYTEK Aurora
Our multiparametric analysis expertise includes spectral flow. With capabilities to detect up to 40 parameters and a higher flow rate, the CYTEK Aurora enables high-throughput cytometry great for clinical sample analysis.
The Lake Forest Expertise
ICS Customized for Your Clinical Studies
Our cytokine assay services measure the percentage of cytokine-producing cells and provide information about the cellular subpopulation expressing cytokines of interest. Applied in vaccine development, oncology, and autoimmunity programs across all clinical phases.
The Lake Forest Expertise
Global Lab Footprint, One Consistent Standard
We offer flow cytometry services across North America, Europe, China, and Australia — aligned with global clinical study needs. Our GCLP environment, CAP accreditations, and CLIA certifications provide rigorous quality assurance oversight at every site.
The Lake Forest Expertise
Receptor Occupancy Assays from Ground Up to Transfer
Our scientists develop custom RO assays from the ground up or transfer already-designed assays to our labs. RO assays generate pharmacodynamic (PD) biomarker data coupled to PK profiles to model PK/PD relationships and inform dose selection.
The Lake Forest Expertise
Why teams choose CellCarta for flow cytometry assays
Flow cytometry decisions shape the quality of your clinical data. Insufficient panel sensitivity, misaligned platform selection, or cross-site variability can delay timelines and compromise confidence in your results.
With a specialist flow cytometry CRO, the right platform and assay strategy does more than generate data — it builds the kind of confidence that holds up in every regulatory submission, across every region, through every phase.
Customizing cytometry assays based on your needs: example with RO assays
Different methodologies can be used to investigate the binding capabilities of biotherapeutics depending on the reagents available. Whether you need a custom receptor occupancy assay, a receptor binding assay, or a functional readout approach, CellCarta has the capabilities to support all three.
As a specialist flow cytometry CRO, we harness the potential of multiparametric flow cytometry, including conventional and spectral flow, to deliver both qualitative and quantitative analyses of clinical samples. Our services are provided in a GCLP environment, with CAP accreditations and CLIA certifications, across labs in North America, Europe, China, and Australia.
Competing vs. non-competing antibodies
Competing antibodies bind the target where the drug normally binds (free receptor), while non-competing antibodies bind a different site (total receptor). The ratio provides the level of receptor occupancy of the drug product.
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Saturated vs. non-saturated with direct drug detection
Half the sample is saturated with the drug product; the other half is not. The drug is detected via secondary antibody staining — particularly useful when monoclonal antibodies to the receptor are unavailable.
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Saturated vs. non-saturated with functional readout
Following in vitro stimulation, cytokine production is monitored as a functional correlate of RO level rather than direct drug detection. Ideal for measuring RO of bi-modal antibodies.
03
Built for reliability, our multi-site flow cytometry infrastructure ensures that results generated from our RO, ICS, and flow cytometry services are of the highest quality. With rigorous quality control in accordance with relevant regulatory requirements — including GCLP — and established assay transfer capabilities across our globally harmonized labs, we maintain consistency even in the face of shifting study timelines. This means fewer disruptions, stronger data integrity, and a CRO partner your teams can depend on at every stage.
Scientist-Led Flow Cytometry Expertise You Can Trust
Quality starts with people. Our immunologists and flow cytometry experts have many years of experience in customizing ICS and RO assays for specific clinical trials — including careful optimization of assay parameters to guarantee reliable results. Regardless of the stimulation used (proteins, peptides, or antigen-presenting cells), we provide an accurate profiling of cellular subsets in your samples. Leveraging our bioinformatic capabilities and CellEngine® Software alongside complementary assays such as ELISpot, we deliver a complete profile of antigen-specific responses that holds up across all our globally accredited flow cytometry labs.
As a specialist flow cytometry CRO, we harness the potential of multiparametric flow cytometry, including spectral flowand the CYTEK Aurora platform, to deliver both qualitative and quantitative analyses of clinical samples. Our services are provided in a GCLP environment, with CAP accreditations and CLIA certifications, across labs in North America, Europe, China, and Australia.
✔ CAP-accredited & CLIA-certified
✔ GLP environment
✔ Globally harmonized
✔ Conventional & spectral flow
Spectral Flow Cytometry with CYTEK Aurora
Our multiparametric analysis expertise includes spectral flow. With capabilities to detect up to 40 parameters and a higher flow rate, the CYTEK Aurora enables high-throughput cytometry great for clinical sample analysis.
ICS Customized for Your Clinical Studies
Our cytokine assay services measure the percentage of cytokine-producing cells and provide information about the cellular subpopulation expressing cytokines of interest. Applied in vaccine development, oncology, and autoimmunity programs across all clinical phases.
Receptor Occupancy Assays from Ground Up to Transfer
Our scientists develop custom RO assays from the ground up or transfer already-designed assays to our labs. RO assays generate pharmacodynamic (PD) biomarker data coupled to PK profiles to model PK/PD relationships and inform dose selection.
Global Lab Footprint, One Consistent Standard
We offer flow cytometry services across North America, Europe, China, and Australia — aligned with global clinical study needs. Our GCLP environment, CAP accreditations, and CLIA certifications provide rigorous quality assurance oversight at every site.
The CellCarta Flow Cytometry Expertise
Why teams choose CellCarta for flow cytometry assays
Spectral flow cytometry & conventional platforms
CYTEK Aurora spectral flow and conventional cytometers, matched to your panel complexity and sample volume.
Dedicated PM & PS for each project
CYTEK Aurora spectral flow and conventional cytometers, matched to your panel complexity and sample volume.
Trusted by start-ups & large pharma
Extensive experience customizing flow cytometry assays for biotech and top 20 pharma programs
Worldwide lab footprint
Labs in North America, Europe, China, and Australia — harmonized protocols across every site, every study
All assays
Cell sorting services to specialized assays like ICS and receptor occupancy assays
Competing vs. non-competing antibodies
Competing antibodies bind the target where the drug normally binds (free receptor), while non-competing antibodies bind a different site (total receptor). The ratio provides the level of receptor occupancy of the drug product.
01
Saturated vs. non-saturated with direct drug detection
Competing antibodies bind the target where the drug normally binds (free receptor), while non-competing antibodies bind a different site (total receptor). The ratio provides the level of receptor occupancy of the drug product.
02
Saturated vs. non-saturated with functional readout
Following in vitro stimulation, cytokine production is monitored as a functional correlate of RO level rather than direct drug detection. Ideal for measuring RO of bi-modal antibodies.
03
